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Specific Targeting of Breast Cancer Cells with Antibodies Conjugated Gold Nanoparticles

[ Vol. 8 , Issue. 3 ]

Author(s):

Zahrah Alhalili, Joe Shapter*, Daniela Figueroa and Barbara Sanderson   Pages 217 - 225 ( 9 )

Abstract:


Background and Objective: Gold nanoparticles (AuNP) conjugated with either EpCAM or TARP antibodies and an anticancer agent, paclitaxel (PTX), for tumour targeting and therapy were synthesised using a simple chemistry.

Methods: The AuNP surface was functionalised using a two-step modification approach. The conjugates were characterised using Transmission Electron Microscopy (TEM) and infrared spectroscopy.

Results: The cytotoxicity assay of T47D cells treated with only antibodies conjugated to the gold nanoparticles did not show any cytotoxicity to the cells, which indicates these nanoconjugates are suitable for intracellular delivery of anticancer drugs.

Conclusion: When using AuNPs with antibodies and the cancer chemotherapy agent PTX attached simultaneously to the functionalised AuNPs, the reduction of cell viability was significantly higher compared to PTX-thiol-AuNPs conjugate system where no antibodies were used.

Keywords:

Gold nanoparticles, paclitaxel, EpCAM, TARP, antibodies, cytotoxicity, drug delivery.

Affiliation:

Flinders Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, Flinders University, Adelaide, SA 5001, Flinders Centre for NanoScale Science and Technology, School of Chemical and Physical Sciences, Flinders University, Adelaide, SA 5001, Medical Biotechnology, School of Medical Science and Technology, Flinders University, Adelaide, SA 5001, Medical Biotechnology, School of Medical Science and Technology, Flinders University, Adelaide, SA 5001

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